The cultivation of algae provides excellent perspectives for renewable energy production and as a source of 'green' products. Algae cultivation can thus contribute substantially to a reduction of CO 2 emissions. This paper describes systems used to cultivate algae from recycle water bottle for biofuel production. For the better quality of Algae cultivation it required a treatment of Nutrients. In this paper we discussed about the construction of Algae bioreactor from recycle water bottle and the treatments of nutrients (Ammonium sulphate, Urea) which required for cultivation of Algae. Result shows that the Algae cultivated from this Algae bioreactor from recycle water bottle is containing large amount of oil than the Algae grow in a rives, wells, ponds etc.