Determining of blood types is very important during emergency situation before administering a blood transfusion. Presently, these tests are performed manually by technicians, which can lead to human errors. Determination of the blood types in a short period of time and without human errors is very much essential. This paper presents a new methodology for determine blood group by taking image of blood sample contend added chemical such as Anitclonal A, B, D and by processing this image in raspberry pi 3 we can get the blood group. Phenotyping based on Raspberry pi and python 3.5 for detection of blood group using image processing. The image processing techniques such as thresholding and morphological operations are used for the basic operation on the images. By using the standalone system based on the raspberry pi we can easily take the image of blood sample process it and the result will be shown on the LCD. Depending on the agglutination rate we can classify the blood group. Thus, the developed automated method determines the blood type using image processing techniques. The developed method is useful in emergency situation and also used when number of people are large.