With the increased use of composites in various sectors as a lightweight material exhibits high strength to weight ratio with tailor made properties. It becomes necessary to focus on various joining methods and different types of composite joints and their strength. The present study aims to improve the mechanical strength of single lap joint of composite material comprises of E-glass fibers and nano modified adhesive. Epoxy adhesive has been modified by dispersing Graphene Oxide (GO) to investigate the possibility of enhancement in the fatigue strength and fracture resistance of the single lap joint. Modified Hummer's method has been used for synthesis of Graphene Oxide. Experimental investigations have been carried out for comparison of tensile and fatigue strength which shows significant improvement in the number of failure cycles for 0.25 wt.% and 0.75 wt.% GO concentrations respectively as compared to neat adhesive. Tension test results showed a significant increase in the fracture toughness of the joint due to addition of GO nanoparticles. There has been 33% and 19% increase in fracture toughness in 0.25 wt.% and 0.75 wt.% GO samples respectively as compared to neat adhesive. Improvement in fracture toughness, among all other nano-reinforcements has been obtained using GO, mainly because of its better capability of deviating the crack growth path to the longer path causing the final failure to retard and consequently improving mechanical properties of the adhesive for the tensile and fatigue strength parameters. © 2021 The Author(s). Published by IOP Publishing Ltd.