A simple extractive spectrophotometric method has been developed for estimation of pefloxacin in both pure and pharmaceutical dosage forms. The method is based on the formation of ion-pair complexes of the drug with four acid dyes namely bromothymol blue (BTB), bromocresol green (BCG), bromophenol blue (BPB), bromocresol purple (BCP) in acidic buffer solutions followed by their extraction in orgnaic solvent (Chloroform). The absorbance of the organic layer was measured at their respective wavelength of maximum absorbance against the corresponding reagent blank. The method has been statistically evaluated and is found to be precise and accurate.