Introduction: In case of internal disasters, such as fire in hos-pitals, health services to the community are severely hampered with the additional morbidity of victims, such as employees and visitors present when the disaster strikes. Risk assessment and fire preparedness are most crucial proactive measures to prevent fire disasters and minimize the loss in a hospital; however, scanty studies are available on this topic. Materials and methods: This cross-sectional study was conducted at a multispecialty hospital in Mumbai, Maharashtra (India), during March–April 2014. Fire-Safety Preparedness Framework (FSPF) was designed with four domains (risk and vulnerability assessment, response mechanisms and strate-gies, preparedness plan and information management) for evaluation of fire safety preparedness of hospital employees. Baseline variables were summarized; instrument was tested for reliability using Cronbach's alpha and content validity through review by experts. The number of correct responses for each question was further analyzed across the type of employee. Results: The instrument showed high reliability (Cronbach's alpha = 0.89, p-value ≤ 0.01) and content validity. A total of 207 employees (mean age 32 ± 8.3 years, 63% females) consented and participated in the study. Out of 20 questions, awareness was high (≥ 90%) only for three questions from " Response mechanism and strategies " domain. For the remain-ing questions, awareness was moderate to low. The awareness varied highly with the type of employee. Conclusion: The FSPF is a reliable tool for application in the Indian context for hospital employees. Disaster preparedness training and drill need to involve employees from all depart-ments as awareness levels varied highly with type of employee.