The deoiled Jatropha cake obtained from biodiesel plant gives the sufficient quantity of bioethanol required for transesterification reaction (1). The chemical and enzyme pre-treatments followed by fermentation with saccromycesscervesse on optimized parameters gives the maximum yield. Bioethanol from fermented substrate was separated byvacuum distillation on 30P 0 Pc and 1 atmosphere . The sugar content increased by chemical treatment is 30g/lit results14g/lit of ethanol. The sugar content obtained by saccrification 60g/lit results 25g/lit of ethanol. The concentration ofobtained bioethanol was analysed by UV spectrophotometer.