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Spectrophotometric simultaneous determination of diclofenac potassium B.P. and thiocolchicoside in combined tablet dosage form by absorption corrected method and area under curve method
M.U. Tryambake, S.N. Savakhande,
Published in
2011
Volume: 7
   
Issue: 2
Pages: 182 - 185
Abstract
A simple, economical, precise and accurate method for simultaneous determination of Diclofenac potassium (DICLO K) and Thiocholchiside (THIO) in combined tablet dosage form has been developed. The first method is based on Absorption corrected and second method is Area Under Curve (AUC) Spectrophotometry. The first method was based on the absorption corrected method in which DICLO K and THIO exhibit λmax at 264.99 nm and 373.84 nm, respectively in methanol. Quantitative estimation of THIO was carried out by subtracting the absorption due to DICLO K at 333.74 nm using experimentally calculated absorption factor and wavelength ranges of 252.56-260.59 nm and 278.51-285.53 nm were selected to determine DICLO K and THIO by AUC method respectively in combined formulations. Beer's law is obeyed in the concentration range of 25-75 μg mL-1 for DICLO K and 4-12 μg mL-1 for THIO for both the methods. The % assay for commercial formulation was found to be in the range 99.01 - 100.89 % for DICLO K and 98.91 - 101.72 % for THIO by the proposed methods. These methods were validated with respect to linearity, precision and accuracy as per ICH analytical method development guidelines. Recovery was found in the range of 98.16 - 100.02 % for DICLO K and 98.56 - 99.81 % for THIO by absorption corrected method and 98.56-99.87% for DICLO K and 99.68-100.21% for THIO by AUC method respectively.
About the journal
JournalInternational Journal of Pharmaceutical Sciences Review and Research
ISSN0976044X