The present study was aimed to assess the anti-arthritic nature of polyherbal formulation containing Cissampelos Pereira Linn, Pongamia pinnata (Linn) Pierre and Vitex negundo Linn. against Freund's complete adjuvant induced arthritis in rats. The degree of inflammation was evaluated by hind paw sweeling, body weight and haematologicalc study supported by histopathology of ankle joints and radiological study. Polyherbal formulation (200 mg/kg, 400 mg/kg and 600 mg/kg) reduces hind paw swelling and body weight alongwith significant improvement in haematological study, while histopathology reveals the significant reduction in mononuclear infiltration, pannus formtion and bone erosion. The radiological pictures of the joints particularly metatarsal, phalanges and the ankle joint space of polyherbal formulation treated group animals showed protective effect against adjuvant induced arthritis.