An ethnobotanical survey was carried out on the use of medicinal plants in Satpuda region of Dhule and Jalgaon districts of Maharashtra. The information was gathered from Pawara, Bhil and Pardhi tribes using an integrated approach of botanical collections, group discussions and interviews with questionnaire during 2004-2005. Among 50 informants interviewed, 8 were tribal practitioners. A total of 67 medicinal plant species distributed in 37 families are documented. In most of the cases, fresh part of the plant was used for the preparation of medicine. These tribal people still depend on the medicinal plants to cure their diseases and disorders in Satpuda forest region. The documented ethnomedicinal plants were mostly used to cure wound infections, skin infections, stomachache, fever, cough, diabetes, diuretics, diarrhoea, eye infections and general weakness.