MIT-World Peace University (MIT-WPU) is a top educational institute located in Pune, India. It is recognized within&nbsp;UGC under Government of Maharashtra Act No. XXXV 2017.&nbsp;

It is part of the MIT group of institutions (established in 1983) which covers 10+ campuses across India and has more than 50,000+ students enrolled across various disciplines at a given point of time. The institute houses 15 schools ranging from Engineering &amp; Technology, Design, Pharmacy, Commerce, Governance among others. It offers undergraduate, postgraduate and PhD programs among different disciplines.&nbsp;

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MIT World Peace University

A reliable, selective and sensitive stability-indicating RP-HPLC assay was established for the quantitation of bromazepam (BMZ) and one of the degradant and stated potential impurities; 2-(2-Amino-5-bromobenzoyl) pyridine (ABP). The assay was accomplished on a C18 column (250 mm × 4.6 mm i.d., 5 ?m particle size) and utilizing methanol-water (70: 30, v/v) as the mobile phase, at a flow rate of 1.0 ml min-1. HPLC detection of elute was obtained by a photodiode array detector (DAD) which was set at 230 nm. ICH guidelines were adhered for validation of proposed method regarding specificity, sensitivity, precision, linearity, accuracy, system suitability and robustness. Calibration curves of BMZ and ABP were created in the range of 1 16 ?g mL-1 with mean recovery percentage of 100.02 1.245 and 99.74 1.124 and detection limit of 0.20 ?g mL-1 and 0.24 ?g mL-1 respectively. BMZ stability was inspected under various ICH forced degradation conditions and it was found to be easily degraded in acidic and alkaline conditions. The results revealed the suitability of the described methodology for the quantitation of the impurity (ABP) in a BMZ pure sample. The determination of BMZ in pharmaceutical dosage forms was conducted with the described method and showed mean percentage recovery of 99.39 1.401 and 98.72 1.795 (n = 6), respectively. When comparing the described procedure to a reference HPLC method statistically, no significant differences between the two methods in regard to both accuracy and precision were found.

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PLoS ONE

Development and validation of a stability indicating RP-HPLC-DAD method for the determination of bromazepam

Exogenous or external antioxidants are required in the form of a food supplements and/or medicines to protect the body from oxidative stress and ultimately from heart disease, stroke, aging and other risks specially cancer. A liquid polyherbal preparation was developed as Saptarangi Plus Kadha which contains three medicinally important herbs such as Salacia chinensis, Curcuma longa and Tinospora cordifolia. The antioxidant activity of Saptarangi Plus Kadha and individual extracts of the said three herbs were carried out by antioxidant capacity assays such DPPH (1, 1-diphenyl-2-picrylhydrazyl radical), FRAP (Ferric reducing antioxidant power) and H2O2 scavenging. The absorbance was measured on UV spectrophotometer at required wavelengths and IC50 values were calculated for Saptarangi Plus Kadha and individual extracts. The individual herbs in combination showed a good synergistic antioxidant activity in SK. Hence, the results are superior for Saptarangi Plus Kadha over individual extracts of the said three herbs. Thus the results of the study proved that Saptarangi Plus Kadha has synergistic antioxidant potential than individual extracts of the said three herbs which indicates further exploration of research work.

Research Journal of Pharmacy and Technology

Synergistic antioxidant activity of a polyherbal preparation

A simple, rapid validated stability indicating HPTLC method for estimation of Salmeterol xinafoate was successfully developed. This method is based on HPTLC separation followed by UV detection at 252 nm. The separation was carried out on Merck TLC aluminium sheets precoated with silica gel 60F254 using Chloroform: Methanol: Ammonia (7:3:0.5 v/v/v) as a mobile phase and scanning was done by using TLC Scanner III. Salmeterol xinafoate gave well defined and sharp peak at Rf 0.52 ± 0.05 at 252 nm. Calibration curve was linear in range 1000-3000 ng/band for Salmeterol xinafoate. Stress degradation study includes hydrolysis under different pH, oxidation, thermal and photolytic conditions. The suitability of this HPTLC method for quantitative estimation of Salmeterol xinafoate was proved by validation in accordance with requirements of ICH guidelines Q2A (R1).

International Journal of Pharmaceutical Quality Assurance

Development and validation of stability indicating HPTLC method for estimation of Salmeterol xinafoate

Indo American Journal of Pharmaceutical Research

4. Preparation, Characterization and Determination of Antioxidant Activity of Anterdhum Padhati Mashi (APM) and Bahirdhum Padhati Mashi (BPM) of Cocos Nucifera Husk

A simple and sensitive UV spectrophotometric method was developed and validated for the determination of pregabalin in bulk & pharmaceutical formulations. The method was linear in the range of 6-14 $\mu$g/ml with a correlation coefficient of 0. 9986. There is no generally accepted method for the determination of pregabalin. The absorbance was measured at 210 nm. The method was validated with respect to accuracy, precision, Stability, limit of detection and limit of quantitation. This method is found to be simple, specific, precise, accurate, reproducible and low cost UV Spectrophotometric method.

International Journal of PharmTech Research

Development and validation of UV spectrophotometric method of pregabalin in bulk and pharmaceutical formulation

Simple, precise, rapid and accurate methods for simultaneous determination of Lamivudine, Zidovudine and Nevirapine in combined tablet dosage form have been developed. Method is based on Ratio spectra derivative and methanol is used as solvent. The amplitudes at 229.77 nm, 316.40 nm and 341.76 nm of the first derivative of ratio spectra were selected to determine Lamivudine, Zidovudine and Nevirapine, respectively by ratio derivative method. Beer's law is obeyed in the concentration range of 6-18 $\mu$g/ml, 12-36 $\mu$g/ml and 8-24 $\mu$g/ml for Lamivudine, Zidovudine and Nevirapine, respectively by Ratio Derivative method. The % assay in commercial formulation was found to be in the range 99.01 - 100.90 % for Lamivudine, 99.3 - 101.11% for Zidovudine and 98.91 - 101.72 % for Nevirapine by the proposed method. The method was validated with respect to linearity, precision and accuracy. Recovery was found in the range of 99.36 - 100.23 % for Lamivudine, 98.91 - 101.32% for Zidovudine and 99.0 - 100.4 % of Nevirapine by ratio derivative method. The method developed is simple, economical, precise and accurate and can be used for routine quality control of combined tablets.

International Journal of Pharmaceutical Research

Simultaneous estimation of Lamivudine, Zidovudine and Nevirapine by ratio derivative spectroscopic method

Three simple, economical, precise and accurate UV Spectrophotometric methods have been developed for the estimation of Mycophenolate sodium in bulk and pharmaceutical formulations. Method A involves measurement of absorbance of solution at 250.23 nm in methanol. Method B involves measurement of integrated area under curve in the wavelength range of 248.07 nm - 250.03 nm and is used to construct calibration curve. Method C is first order derivative spectroscopy method, in which first order derivative amplitude at 258.42 nm is measured. Linearity for detector response was observed in the concentration range of 10 - 50 $\mu$g/ml for all the three methods in methanol. The results of analysis have been validated as per ICH analytical method validation guidelines. Accuracy, precision and recovery studies were carried which confirmed the validity of the proposed methods. {\textcopyright} RJPT All right reserved.

Development and Validation of UV Spectrophotometric Methods for Estimation of Mycophenolate Sodium in Bulk and Pharmaceutical Formulations

Two methods for the simultaneous estimation of atenolol and hydrochlorothiazide in combined tablet dosage form have been developed. The first method is the Q-analysis method, based on absorbance ratio at two selected wave lengths 232.0nm (Iso-absorptive point) and 270.0nm ($\lambda$max of hydrochlorothiazide). The second method is the first order derivative spectroscopy using 214.0nm (zero cross for atenolol) and 241.0nm (zero cross for hydrochlorothiazide). The linearity was obtained in both the methods in the concentration range of 1-30$\mu$g/ml and 1-40$\mu$g/ml for atenolol and hydrochlorothizide, respectively. These methods are simple, accurate and results of analysis have been validated statistically and by recovery studies.