MIT-World Peace University (MIT-WPU) is a top educational institute located in Pune, India. It is recognized within&nbsp;UGC under Government of Maharashtra Act No. XXXV 2017.&nbsp;

It is part of the MIT group of institutions (established in 1983) which covers 10+ campuses across India and has more than 50,000+ students enrolled across various disciplines at a given point of time. The institute houses 15 schools ranging from Engineering &amp; Technology, Design, Pharmacy, Commerce, Governance among others. It offers undergraduate, postgraduate and PhD programs among different disciplines.&nbsp;

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MIT World Peace University

We have designed and synthesized (Z)-2-(5-methyl-2-oxo-2H-chromen-7-yl) oxy)-N'-(2-oxoindolin-3-ylidin) acetohydrazide derivatives by reacting 7-hydroxy-4-methyl-coumarin and substituted isatin to afford 12 substituted coumarin acetohydrazide derivatives. The synthesized compounds of coumarin acetohydrazide derivatives were designed and evaluated to study for their possible interactions as DNA gyrase inhibitors. All the synthesized coumarin acetohydrazide compounds have been characterized by spectral analysis IR, 1H NMR and mass spectroscopy. The compounds have been evaluated for In vitro antibacterial and antifungal activity against S. aureus, B. subtilus, E. coli, P. aeruginosa and fungi C. albicans and A. niger. In case of Gram positive bacteria and Gram negative bacteria Compound P5C, M5C, C5C exhibited significant activity. Compounds P5N, M5N, C5N shown moderate activity. Compound P5C, M5C, C5C shown significant Antifungal activity against C. albicans and A. niger. Compounds P5C, M5C, C5C are found to exert significant antibacterial as well as antifungal activity and can serve as potential compound against infectious diseases in future.

School of Pharmacy

Pharmacy

World University root node

Research Journal of Pharmacy and Technology

Design, synthesis and antibacterial, antifungal activity of some coumarin acetohydrazide derivatives

Aim: To design, synthesize, anti-inflammatory \&amp; anticonvulsant activity of substituted coumarin acetohydrazide derivatives. Study Design: Experimental work related to Anti-inflammatory \&amp; Anticonvulsant activity. Methodology: Coumarin acetohydrazide derivatives were synthesized by reacting substituted isatin with 7-hydroxy-4-methyl-coumarin in glacial acetic acid to determine their anti-inflammatory \&amp; anticonvulsant activities. Molecular docking study was performed against the COX enzyme for anti-inflammatory activity \&amp; carbonic anhydrase II enzyme for anticonvulsant activity. Anti-inflammatory activity was done by carrageenan induced paw edema method in rats at a dose of 200 mg/kg body weight. Anticonvulsant activity was studied in rats at a dose of 200 mg/kg body weight; in the maximal electroshock (MES) induced seizures model. Results: (Z)-2-(4,8-dimethyl-2-oxo-2H-chromen-7-yl)oxy)-N-(5-nitro-2-oxoindolin-3 -ylidine) aceto- hydrazide and (Z)-2-(4-methyl-2-oxo-2H-chromen-7yl) oxy)-N-(1-methyl-2-oxoindolin-3-ylidene) acetohydrazide showed the highest anti-inflammatory activity as compared to Celecoxib after 5 h with 71.94 to 77.96 \% inhibition. Most of the compounds displayed anticonvulsant activity in the MES screen at a dose 200 mg/kg. Out of twelve compounds (Z)-N-(5-chloro-2-oxoindolin-3-ylidene)-24(4-methyl-2-oxo-2H-chromen-7-y l) oxy) acetohydrazide and (Z)-2-((4-methyl-2-oxo-2-Hchromen-7yl) oxy)-N-(1-methyl-2-oxoindolin-3-ylidene) acetohydrazide shown most significant activity with remarkable protection (67\%) against MES induced convulsions. The structure-activity relationship concluded valuable pharmacophoric information, that the substitution on isatin ring has a significant effect on preventing inflammation \&amp; the seizure formation. Conclusion: From the molecular docking study \&amp; biological activity determines that all synthesized coumarin acetohydrazide derivatives shows anti-inflammatory activity as compared to Celecoxib \&amp; anticonvulsant activity as compared to Phenytoin.

JOURNAL OF PHARMACEUTICAL RESEARCH INTERNATIONAL

Design, Synthesis, Anti-inflammatory \&amp; Anticonvulsant Activity of Substituted Heterocyclic Compounds

Three simple, economical, precise and accurate UV Spectrophotometric methods have been developed for the estimation of Mycophenolate sodium in bulk and pharmaceutical formulations. Method A involves measurement of absorbance of solution at 250.23 nm in methanol. Method B involves measurement of integrated area under curve in the wavelength range of 248.07 nm - 250.03 nm and is used to construct calibration curve. Method C is first order derivative spectroscopy method, in which first order derivative amplitude at 258.42 nm is measured. Linearity for detector response was observed in the concentration range of 10 - 50 $\mu$g/ml for all the three methods in methanol. The results of analysis have been validated as per ICH analytical method validation guidelines. Accuracy, precision and recovery studies were carried which confirmed the validity of the proposed methods. {\textcopyright} RJPT All right reserved.